Strong ZsGreen expression in F 1 seeds was observed after recombination when ZsGreen was driven by the AtUbq10 promoter. The incapability to observe ZsGreen fluorescence was attributed to the activity of the AtUbq3 pro. In crosses between Cre recombinase lines and transgenic lines harboring a lox -excision reporter cassette with ZsGreen driven by the AtUbq3 promoter after Cre/ lox -mediated recombination, we observed complete excision of the lox -flanked intervening DNA sequence between the AtUbq3 pro and the ZsGreen coding sequence in F 1 progeny upon genotyping but no ZsGreen expression in F 1 seeds or seedlings. To activate transgenes exclusively in a hybrid upon fertilization, we evaluated a Cre/ lox -mediated gene activation system with the Cre recombinase expressed by either AtRps5a or AtDD45 promoters that showed activity in egg cells and young embryos. Genetic engineering ideally results in precise spatiotemporal control of transgene expression. Let Surfer automate the tedious part of your work and power creativity with hard data.Expression of Cre recombinase by AtRps5a pro or AtDD45 pro enabled Cre/ lox -mediated recombination at an early embryonic developmental stage upon crossing, activating transgenes in the hybrid cowpea and tobacco. With Surfer’s smart algorithms, you will quickly find the best content opportunities, create a content strategy for months ahead, and write compelling pages based on data-driven guidelines. Thousands of content, SEO, and marketing specialists use Surfer to accelerate their digital marketing. We believe marketing results and user satisfaction go hand in hand, and you should never sacrifice one for the other. Surfer’s mission is to support everyone in delivering the best, most relevant content and raise the quality of the web. Surfer is the Content Intelligence tool that unifies content strategy, creation, and optimization into one smooth process-to help content teams grow brands, organic traffic, and revenue. Streamline your whole content creation research process without ever leaving Google results page.ĭata for the extension are provided by Surfer:. You can also create keyword collections that will allow you to easily bookmark important keywords without having to google them separately every time. Keyword Surfer allows you to see search volume directly in Google search results.įor every new search in Google, you'll uncover valuable insights on search volume, CPC, keyword suggestions, related terms, visibility metrics, and on-page data. Keyword Surfer allows you to generate keyword ideas and see search volumes directly in Google search results.
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